Size  100ul
Cat code  220021
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Clone Number  5C5-C2-A5
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  86kDa
Sensitivity  The antibody detects endogenous level of total Ku80 protein.
Immunogen  Purified recombinant fragment of human XRCC5 expressed in E. Coli.
UniProt Number  P13010
GeneBank ID  NM_021141.3
Subcellular location  Nucleus. Nucleus ? nucleolus. Chromosome
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  86 kDa subunit of Ku antigen ; ATP dependent DNA helicase 2 subunit 2 ; ATP dependent DNA helicase II 80 kDa subunit ; ATP dependent DNA helicase II 86 Kd subunit ; ATP dependent DNA helicase II ; ATP-dependent DNA helicase 2 subunit 2 ; ATP-dependent DNA helicase II 80 kDa subunit ; CTC box binding factor 85 kDa ; CTC box-binding factor 85 kDa subunit ; CTC85 ; CTCBF ; DNA repair protein XRCC5 ; KARP 1 ; KARP1 ; Ku 80 ; Ku autoantigen 80kDa ; Ku80 ; Ku86 ; Ku86 autoantigen related protein 1 ; KUB 2 ; KUB2 ; Lupus Ku autoantigen protein p86 ; NFIV ; Nuclear factor IV ; Thyroid lupus autoantigen ; Thyroid-lupus autoantigen ; TLAA ; X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) ; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) ; X-ray repair cross-complementing protein 5 ; Xray repair complementing defective repair in Chinese hamster cells 5 ; XRCC 5 ; XRCC5 ; XRCC5_HUMAN ;
Gene_symbol  KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Summary  The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3´-5´ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5´-deoxyribose-5-phosphate lyase (5´-dRP lyase), by catalyzing the beta-elimination of the 5´ deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5´-dRP activity, and allows to ´clean´ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Application Images :  
Western blot detection of Ku80 antibody in A549, MCF7 and Hela cell lysates using Ku80 antibody(dilution 1:500).Predicted band size:80KDa.Observed band size:80KDa.
Immunocytochemistry of HeLa cells using anti-Ku80 antibody diluted 1:100.