Size  100ug
Cat code  200056
Species Cross-reactivity  H,M
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  90kDa
Sensitivity  This antibody detects endogenous levels of UHRF1 and does not cross-react with related proteins
Immunogen  Purified recombinant human UHRF1 protein fragments expressed in E.coil.
UniProt Number  Q96T88
GeneBank ID  NM_013282.3
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  Np95; hNP95; ICBP90; RNF106; FLJ21925; MGC138707
Gene_symbol  Np95; hNP95; ICBP90; RNF106
Summary  This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
UniPort summary  Function  Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5´-CCAAT-3´ box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
Application Images :  
Western blot detection of UHRF1 antibody in Mouse heart,293T,HelaNE&Hela cell lysates using UHRF1 antibody (1:1000 diluted).
Predicted band size: 90KDa
Observed band size: 90KDa
Immunocytochemistry of HeLa cells using anti- UHRF1 (N-terminus) antibody diluted 1:200.