Size  100ug
Cat code  200678
Species Cross-reactivity  H,M
Key Application  WB,ICC,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  69kDa
Sensitivity  This antibody detects endogenous levels of DLAT and does not cross-react with related proteins.
Immunogen  Purified recombinant human DLAT protein fragments expressed in E.coli.
UniProt Number  P10515
GeneBank ID  NM_001931.4
Subcellular location  Mitochondrion matrix.
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  70 kDa mitochondrial autoantigen of primary biliary cirrhosis;
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex;
Dihydrolipoamide S Acetyltransferase;
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex;
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial;
DLAT;
DLTA;
E2;
E2 component of pyruvate dehydrogenase complex;
M2 antigen complex 70 kDa subunit;
mitochondrial;
ODP2_HUMAN;
PBC;
PDC E2;
PDC-E2;
PDCE2;
Pyruvate dehydrogenase complex component E2;
Pyruvate dehydrogenase complex E2 subunit;
Gene_symbol  DLTA; PDCE2; PDC-E2 
Summary  This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood
UniPort summary  Function  The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Application Images :  
Western blot detection of DLAT in Jurkat,A549,U251,F9,LNCAP&Hela cell lysates using DLAT antibody (1:1000 diluted).
Predicted band size:69KDa.
Observed band size:69KDa
Immunocytochemistry stain of Hela using DLAT antibody (1:300).
Immunoprecipitation analysis of Hela cell lysates using DLAT antibody