Size  100ug
Cat code  200503
Species Cross-reactivity  H
Key Application  WB,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  58kDa
Sensitivity  This antibody detects endogenous levels of Ikaros(C-terminus) and does not cross-react with related proteins.
Immunogen  Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.
UniProt Number  Q13422
GeneBank ID  NM_006060
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  IK1; LYF1; hIk-1; IKAROS; PRO0758; ZNFN1A1; Hs.54452
Gene_symbol  IK1; LYF1; hIk-1; IKAROS; PRO0758; ZNFN1A1; Hs.54452
Summary  Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher
affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the
enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene
during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and
HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex
(PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells.
Confers early temporal competence to retinal progenitor cells (RPCs).
UniPort summary  Function  Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity)
Application Images :  
Western blot detection of Ikaros(C-terminus) antibody in Ramos&Jurkat cell lysates using Ikaros(C-terminus) antibody (1:1000 diluted).
Predicted band size: 58KDa
Observed band size: 58KDa.
Immunoprecipitation analysis of Hela cell lysate using IKZF1 antibody.