Size  100ug
Cat code  200790
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  60kDa
Sensitivity  This antibody detects endogenous levels of SMAD2 and does not cross-react with related proteins.
Immunogen  Purified recombinant human SMAD2 protein fragments expressed in E.coli.
UniProt Number  Q15796
GeneBank ID  NM_001003652
Subcellular location  Cytoplasm. Nucleus.
Formulation  ascites
Altername  hMAD 2;hMAD-2;hSMAD2;JV18 1;JV18;JV18;JV18-1;JV181;MAD;MAD;MAD homolog 2;MAD Related Protein 2;Mad-related protein 2;MADH2;MADR2;MGC22139;MGC34440;Mothers Against Decapentaplegic Homolog 2;Mothers Against Decapentaplegic Homolog 2;mothers against DPP homolog 2;OTTHUMP00000163489;Sma and Mad related protein 2;SMAD 2;SMAD;SMAD family member 2;SMAD, mothers against DPP homolog 2;SMAD2;SMAD2_HUMAN.
Gene_symbol  JV18;MADH2;MADR2;JV18-1;hMAD-2;hSMAD2.
Summary  The protein encoded by this gene belongs to the SMAD,a family of proteins similar to the gene products of the Drosophila gene ´mothers against decapentaplegic´ (Mad) and the C.elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.This protein mediates the signal of the transforming growth factor (TGF)-beta,and thus regulates multiple cellular processes,such as cell proliferation,apoptosis,and differentiation.This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein.In response to TGF-beta signal,this protein is phosphorylated by the TGF-beta receptors.The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of this protein into the nucleus,where it binds to target promoters and forms a transcription repressor complex with other cofactors.This protein can also be phosphorylated by activin type 1 receptor kinase,and mediates the signal from the activin.Alternatively spliced transcript variants have been observed for this gene.[provided by RefSeq,May 2012]
UniPort summary  Function  Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and,on formation of the SMAD2/SMAD4 complex,activates transcription.May act as a tumor suppressor in colorectal carcinoma.Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Application Images :  
Western blot detection of SMAD2 antibody in Hela,A431,Jurkat and K562 cell lysates using SMAD2 antibody (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.