Size  100ug
Cat code  200948
Species Cross-reactivity  H,Mk,M,R
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  70kDa
Sensitivity  This antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.
Immunogen  Purified recombinant human RPA70 protein fragments expressed in E.coli.
UniProt Number  P27694
GeneBank ID  NM_002945
Subcellular location  Nucleus.
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername  Dmrpa1;Drosophila Replication Protein A;DRPA;HSSB;Human single stranded DNA binding protein;MST075;MSTP075;p70;REPA1;Replication factor A;Replication factor A protein 1;Replication protein A 70 kDa DNA-binding subunit;Replication protein A 70kDa DNA binding subunit;Replication protein A1 70kDa;Replication protein A1;RF A;RF-A protein 1;RFA;RFA1_HUMAN;RP A;RP-A p70;RPA 70;RPA;RPA1;Single stranded binding protein 70;Single-stranded DNA-binding protein.
Gene_symbol  HSSB;RF-A;RP-A;REPA1;RPA70;MST075.
Summary  null
UniPort summary  Function  Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Application Images :  
Western blot detection of RPA70 antibody in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
Western blot detection of RPA70 antibody in Hela,293T,C6,3T3 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.