Size  100ug
Cat code  200797
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  260kDa
Sensitivity  This antibody detects endogenous levels of CHD4 and does not cross-react with related proteins.
Immunogen  Purified recombinant human CHD4 protein fragments expressed in E.coli.
UniProt Number  Q14839
GeneBank ID  NM_001273
Subcellular location  Nucleus.Cytoplasm cytoskeleton centrosome.
Formulation  Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
Altername  ATP-dependent helicase CHD4,Mi-2 autoantigen 218 kDa protein,Mi2-beta
Gene_symbol  Mi-2b;Mi2-BETA
Summary  The product of this gene belongs to the SNF2/RAD54 helicase family.It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression.Patients with dermatomyositis develop antibodies against this protein.[provided by RefSeq,Jul 2008]
UniPort summary  Function  Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Application Images :  
Western blot detection of CHD4 in K562,HelaNE,HL-60,MOLT-4,Jurkat & 293 cell lysates using CHD4 antibody(1:1000 diluted).Predicted band size:260KDa,Observed band size:260KDa.