Size  100ul
Cat code  200960
Species Cross-reactivity  H,R,Mk
Key Application  WB
Host  Mouse
Clone Number  7D6-A9-D4
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  62kDa
Sensitivity  This antibody detects endogenous levels of FOS and does not cross-react with related proteins.
Immunogen  Purified recombinant human FOS protein fragments expressed in E.coli.
UniProt Number  P01100
GeneBank ID  NM_005252
Subcellular location  Nucleus.Endoplasmic reticulum.Cytoplasm ? cytosol.
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername  Activator protein 1;AP 1;Cellular oncogene c fos;Cellular oncogene fos;FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS);FBJ murine osteosarcoma viral v fos oncogene homolog;FBJ Osteosarcoma Virus;FOS;FOS protein;FOS_HUMAN;G0 G1 switch regulatory protein 7;G0/G1 switch regulatory protein 7;G0S7;Oncogene FOS;p55;proto oncogene c Fos;Proto oncogene protein c fos;Proto-oncogene c-Fos;v fos FBJ murine osteosarcoma viral oncogene homolog.
Gene_symbol  p55;AP-1;C-FOS.
Summary  The Fos gene family consists of 4 members: FOS,FOSB,FOSL1,and FOSL2.These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family,thereby forming the transcription factor complex AP-1 As such,the FOS proteins have been implicated as regulators of cell proliferation,differentiation, and transformation.In some cases,expression of the FOS gene has also been associated with apoptotic cell death.[provided by RefSeq,Jul 2008]
UniPort summary  Function  Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.In the heterodimer,FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.On TGF-beta activation,forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.Has a critical function in regulating the development of cells destined to form and maintain the skeleton.It is thought to have an important role in signal transduction,cell proliferation and differentiation.In growing cells,activates phospholipid synthesis,possibly by activating CDS1 and PI4K2A.This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Application Images :  
Western blot detection of c-Fos antibody in HCT116,COS7 and C6 cell lysates using c-Fos antibody (1：500 diluted).Predicted band size:62KDa.Observed band size:62KDa.
Western blot detection of c-Fos antibody in Hela(TSA treated ) and Hela cell lysates using c-Fos antibody (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.