Size  100ug
Cat code  200411
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  45kDa
Sensitivity  This antibody detects endogenous levels FAS and does not cross-react with related proteins.
Immunogen  Purified recombinant human FAS protein fragments expressed in E.coli.
UniProt Number  P25445
GeneBank ID  NM_000043
Subcellular location  Isoform 1: Cell membrane; Single-pass type I membrane protein 
Isoform 2: Secreted 
Isoform 3: Secreted 
Isoform 4: Secreted
Isoform 5: Secreted 
Isoform 6: Secreted
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Gene_symbol  APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Summary  The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death 
domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
UniPort summary  Function  Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Application Images :  
Western blot detection of FAS(C-terminus) antibody in Jurkat cell lysates using FAS(C-terminus) antibody (1:1000 diluted).
Predicted band size: 45KDa
Observed band size: 45KDa