Size  100ul
Cat code  200306-7E4
Species Cross-reactivity  H,Mk,R,M
Key Application  WB
Host  Mouse
Clone Number  7E4-H6-H6
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgM
Molecular Weight  37kDa
Sensitivity  This antibody detects endogenous levels of GAPDH and does not cross-react with related proteins.
Immunogen  Purified recombinant human GAPDH protein fragments expressed in E.coli.
UniProt Number  P04406
GeneBank ID  NM_002046
Subcellular location  Cytoplasm >> cytosol.Nucleus.Cytoplasm >> perinuclear region.Membrane.Cytoplasm >> cytoskeleton.
Formulation  Ascites containing with 0.02% sodium azide and 50% glycerol.
Altername  38 kDa BFA-dependent ADP-ribosylation substrate;Aging-associated gene 9 protein;BARS-38;cb609;EC 1.2.1.12;G3P_HUMAN;G3PD;G3PDH;GAPD ;GAPDH;GAPDH;Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle;Glyceraldehyde-3-phosphate dehydrogenase;KNC-NDS6;MGC102544;MGC102546;MGC103190;MGC103191;MGC105239;MGC127711 ;MGC88685;OCAS,p38 component;OCT1 coactivator in S phase,38-KD component;Peptidyl-cysteine S-nitrosylase GAPDH;wu:fb33a10.
Gene_symbol  G3PD;GAPD.
Summary  The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
UniPort summary  Function  Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Application Images :  
Western blot detection of GAPDH antibody in K562,3T3,C6 and Hela cell lysates using GAPDH antibody (1:10000 diluted). Predicted band size:37KDa. Observed band siz37KD.
Western blot detection of GAPDH antibody in Jurkat,HepG2,Molt-4,COS7,3T3,C6 and Hela cell lysates using GAPDH antibody (1:10000 diluted).